Thermo Fisher Scientific SMAD4 Polyclonal Antibody
SMAD4 단백질을 인식하는 Thermo Fisher의 토끼 폴리클로날 항체입니다. Western blot과 IHC(P)에서 검증되었으며, 항원 친화 크로마토그래피로 정제되었습니다. 인간, 마우스, 랫트에 반응하며, 1 mg/mL 농도의 액상 형태로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 12–64 of Human Smad4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C for short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2803183 |
Product Specific Information
Purity is >95% by SDS-PAGE.
Target Information
Smad4 is encoded by the Smad4 gene located on chromosome 18 in humans. It is also known as Smad family member 4, Sma- and Mad-related protein 4, or Mothers against decapentaplegic (MAD) homolog 4.
Smad4 contains N-terminal MH1 (MAD homology 1) and C-terminal MH2 (MAD homology 2) domains involved in DNA binding and protein interactions.
Binding of TGF-beta superfamily ligands (including Transforming Growth Factor-beta and bone morphogenetic protein) to their receptors leads to phosphorylation of Smad1, 2, 3, 5, and 8 (R-Smads). These form heterotrimers with Smad4 (co-Smad), which translocate to the nucleus to regulate gene expression.
Smad4 functions as a tumor suppressor by regulating target genes such as Cyclin D1 (downregulation) and collagen (upregulation), thereby inhibiting cell proliferation. Dephosphorylation controls nuclear export and nucleocytoplasmic dynamics of Smads.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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