Thermo Fisher Scientific CD229 Polyclonal Antibody
Rabbit polyclonal antibody recognizing human CD229 (Ly9). Validated for WB and ICC/IF. Suitable for detecting CD229 in lymphocyte-related studies. Supplied as liquid, 1 mg/mL, stored at 2–8°C or -20°C for long-term use.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | An 18 amino acid peptide near the carboxy terminus of human SLAMF3 (CD229, Ly9, UniProt ID: Q9HBG7-1). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | Store at 2–8°C for up to 3 months. For long-term storage, keep at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152689 |
Product Specific Information
- A suggested positive control is 293 cell lysate.
- PA5-21135 can be used with blocking peptide PEP-1249.
Target Information
CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes CD48 and CD224. These receptors regulate cytokine production and cytotoxicity of lymphocytes and NK cells.
High levels of CD229 are found on T and B cells, with expression increasing during maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets, and erythrocytes.
CD229 is also reported on mouse monocytes and NK cells. It interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen-presenting B cells during antigen-dependent immune synapse formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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