Thermo Fisher Scientific ZMIZ1 Polyclonal Antibody
ZMIZ1 단백질을 인식하는 Rabbit Polyclonal Antibody로 Western blot 및 Immunocytochemistry에 적합. 항원 친화 크로마토그래피로 정제되었으며, PBS 버퍼에 0.02% sodium azide 포함. K562 lysate를 양성 대조로 권장하며, 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 15 amino acid peptide near the amino terminus of human ZIMP10 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at −20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154506 |
Product Specific Information
- A suggested positive control is K562 cell lysate.
- PA5-21133 can be used with blocking peptide PEP-1247.
Target Information
Retinoic acid plays a critical role in development, cellular growth, and differentiation and induces the expression of a variety of genes.
RAI17 expression is induced by retinoic acid and is predominantly expressed in heart, brain, and ovaries. Within the brain, highest expression is in the amygdala.
The deduced 1,067-amino acid protein contains an MSX-interacting zinc finger (MIZ) domain, a nuclear localization signal sequence, and two proline-rich regions.
A strong intrinsic transactivation domain is identified in the C-terminal proline-rich region.
RAI17 expression is detected in various cancer cell lines and colocalizes with endogenous androgen receptor (AR) in the nuclei of prostate epithelial cells from human tissue samples.
In human prostate cancer cells, RAI17 increases the transcriptional activity of AR. Studies using sumoylation-deficient AR mutants suggest that this increase of AR activity by RAI17 is dependent upon receptor sumoylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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