Thermo Fisher Scientific WTAP Polyclonal Antibody
WTAP 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, Western blot, ICC/IF, ELISA에 사용 가능. 인간, 마우스, 랫트 반응성. 핵 내 WTAP 단백질 검출에 적합하며, 액상형으로 -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein corresponding to amino acids 212–355 of human WTAP (NP_0048972) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805878 |
Product Specific Information
Immunogen sequence:
DEEVEGMQST ILVLQQQLKE TRQQLAQYQQ QQSQASAPST SRTTASEPVE QSEATSKDCS RLTNGPSNGS SSRQRTSGSG FHREGNTTED DFPSSPGNGN KSSNSSEERT GRGGSGYVNQ LSAGYESVDS PTGSENSLTH QSND
Positive Samples: Rat spleen
Cellular Location: Nucleus, Nucleus speckle, nucleoplasm
Target Information
Wilms' tumor (WT) is an embryonal malignancy of the kidney affecting approximately 1 in 10,000 infants. The Wilms' tumor protein (WT1) binds the DNA sequence GCGGGGGCG and functions as a transcriptional repressor.
WTAP (Wilms tumor 1-associating protein) is a ubiquitously expressed nuclear protein that interacts with WT1 and may regulate mRNA splicing. WTAP localizes to nuclear speckles and regulates the G2/M cell cycle transition by binding to the 3' UTR of cyclin A2, enhancing its stability. Additionally, WTAP inhibits WT1 target gene expression and reduces WT1 DNA-binding ability. Two isoforms of WTAP exist due to alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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