Thermo Fisher Scientific SQSTM1 Monoclonal Antibody (OTI6A7), TrueMAB
인간 SQSTM1 단백질을 타겟으로 하는 Mouse IgG2a 단클론 항체. Western blot, ICC/IF, Flow cytometry에 적합. 동결건조 형태로 제공되며, 재구성 시 1 mg/mL 농도 권장. 유비퀴틴 결합 및 NF-κB 신호 조절 연구에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6A7 |
| Immunogen | Full-length human recombinant SQSTM1 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
SQSTM1 encodes a multifunctional protein that binds ubiquitin and regulates activation of the NF-κB signaling pathway. It acts as a scaffolding/adaptor protein with TNF receptor-associated factor 6 to mediate NF-κB activation in response to upstream signals.
Alternative splicing generates different isoforms. Mutations in SQSTM1 are associated with sporadic and familial Paget disease of bone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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