Thermo Fisher Scientific Phospho-XRCC1 (Thr284) Polyclonal Antibody
인간 XRCC1의 Thr284 인산화 형태를 특이적으로 인식하는 폴리클로날 항체. Western blot, IHC, ICC/IF에 사용 가능. 고순도 액상 형태로 PBS/glycerol 버퍼에 보관. DNA 손상 복구 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human XRCC1 (Accession P18887), corresponding to amino acid residues around phosphorylated Thr284 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2903290 |
Product Specific Information
This antibody detects endogenous levels of XRCC1 only when phosphorylated at Thr284.
Target Information
XRCC1 (X-ray cross complementing factor-1) is involved in DNA base excision repair. It is a 70 kDa protein associated with DNA repair enzymes such as poly (ADP-ribose) polymerase (PARP), DNA Ligase III, and DNA polymerase beta. XRCC1 contains a BRCT domain (BRCA1 carboxyl terminus), which mediates protein-protein interactions, including with DNA ligase III-alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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