Thermo Fisher Scientific p21 Monoclonal Antibody (A8C12)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:400

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

A8C12

Immunogen

Recombinant protein within full length of human p21 aa 1-164/164. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:p21,uniProtId:P38936-1,ncbiNodeId:9606,antigenRange:1-164,antigenLength:164,antigenImageFileName:MA5-50568_p21_P38936-1_House_mouse.svg,antigenImageFileNamePDP:MA5-50568_p21_P38936-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3091234

Product Specific Information

Positive control: Human cervical well-differentiated squamous cell carcinoma tissue, human thyroid carcinoma tissue.

Predicted band size: 16 kDa

Subcellular Location: Nucleus, Cytoplasm.

Target Information

Protein p21 is a tumor suppressor protein which is an inhibitor of Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. P21 is important in the response of cells to genotoxic stress and a major transcriptional target of p53 protein. The occurrence of p21 in the nucleus executes binding and inhibition activity of cyclin dependent kinases Cdk1 and Cdk2, and blocks the transition from G1 phase into S phase or from G2 phase into mitosis after DNA damage, which enables the repair of damaged DNA. In the cytoplasm, p21 protein has an anti-apoptotic effect. P21 is able to bind to and inhibit caspase 3, as well as the apoptotic kinases ASK1 and JNK. P21 exhibits a dual function in carcinogenesis, and acts as a tumor suppressor, prevents apoptosis, and acts as an oncogene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.