Thermo Fisher Scientific Phospho-SMAD5 (Ser463, Ser465) Polyclonal Antibody
SMAD5 단백질의 Ser463/465 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC 등 다양한 응용 가능. Human, Mouse, Rat 반응성. 1 mg/mL 농도의 액상 형태로 제공되며, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human SMAD5 (Accession Q99717), corresponding to amino acid residues around phosphorylated Ser465 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2815855 |
Product Specific Information
This antibody detects endogenous levels of Smad5 only when phosphorylated at S463/465.
Target Information
SMADs are members of the MAD-related family of intracellular proteins that are essential components in the signaling pathways of serine/threonine kinase receptors of the transforming growth factor beta (TGF-β) superfamily.
SMADs are classified as:
- Receptor-regulated SMADs (R-SMADs): SMAD1, 2, 5, 8, 9
- Common-mediator SMAD (Co-SMAD): SMAD4
- Inhibitory SMADs (I-SMADs): SMAD6, 7
SMAD1, 5, 8, and 9 share high sequence homology, and antibodies may cross-react with common sequences. SMAD8 and SMAD9 are often used interchangeably in literature.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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