Thermo Fisher Scientific Rat anti-Mouse IgG1 Secondary Antibody, APC, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

• Biotin
• eFluor 450
• eFluor 570
• eFluor 615
• eFluor 660
• FITC
• HRP
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Custom conjugation available upon request

Product Specific Information

The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and is suitable as a secondary reagent in flow cytometry and microscopy.
It does not recognize other mouse isotypes nor cross-react with rat IgG1 or other rat isotypes.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested by flow cytometric analysis of cells stained with a mouse IgG1 primary antibody
• Recommended usage: ≤ 0.25 µg per test (100 µL final volume)
• Cell number range: 10⁵–10⁸ cells/test
• Antibody should be titrated for optimal performance
• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Anti-Mouse secondary antibodies are affinity-purified with well-characterized specificity for mouse immunoglobulins.
They are useful for detection, sorting, or purification of target antibodies.
Secondary antibodies enhance assay versatility and sensitivity by allowing multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification.
Typically produced by immunizing host animals with pooled immunoglobulins from the target species, followed by purification and optional modifications such as label conjugation or fragmentation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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