Thermo Fisher Scientific F(ab`)2-Goat anti-Mouse IgG (H+L) Secondary Antibody, APC, eBioscience

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Flow cytometry용 APC 결합 F(ab')2 goat anti-mouse IgG(H+L) 2차 항체. Fc 영역 제거로 비특이적 결합 최소화. 651/660 nm 형광 특성, 0.2 mg/mL 농도. 인체 진단용이 아닌 연구용 시약.

카탈로그번호: 17-4010-82
브랜드: Thermo Fisher Scientific
카테고리: Polyclonal Antibodies
판매단위: pk

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Thermo Fisher Scientific 17-4010-82 F(ab`)2-Goat anti-Mouse IgG (H+L) Secondary Antibody, APC, eBioscience 100 ug pk판매 단위 pk ·

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170,100원VAT 포함 187,110원

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Thermo Fisher Scientific · Thermo Fisher Scientific F(ab`)2-Goat anti-Mouse IgG (H+L) Secondary Antibody, APC, eBioscience

Applications and Tested Dilution

Application: Flow Cytometry (Flow)
Tested Dilution: 0.5 µg/test

Product Specifications

항목	내용
Species Reactivity	Mouse
Host/Isotype	Goat / Ig
Class	Polyclonal
Type	Secondary Antibody
Conjugate	APC
Excitation/Emission Max	651 / 660 nm
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage Buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage Conditions	4°C, store in dark, DO NOT FREEZE
Shipping Conditions	Ambient (domestic); Wet ice (international)
RRID	AB_2573203
Target	IgG
Antibody Form	F(ab`)2 Fragment

Product Specific Information

The goat anti-mouse IgG polyclonal antibody reacts with the heavy and light chains of mouse IgG, with minimal cross-reactivity to human, rat, bovine, goat, hamster, and rabbit serum proteins.
Reactivity has been shown to IgG1, IgG2a, IgG2b, and IgG3, with some reactivity to IgM.
This antibody is a F(ab`)2 fragment, containing two Fab domains linked through the hinge region. Removal of the Fc region reduces Fc-mediated binding issues.

This polyclonal antibody has been validated as a secondary reagent for recognizing cells stained with a mouse IgG primary antibody.
Reported application: Flow cytometric analysis.

Recommended Usage

Tested by flow cytometric analysis of normal human peripheral blood cells.
Use ≤ 0.5 µg per test (100 µL final volume).
Optimal cell number: 10⁵–10⁸ cells/test (empirically determined).
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins.
They are useful in detection, sorting, or purification of target proteins.
Secondary antibodies increase versatility and sensitivity by enabling multiple detection systems (e.g., HRP, AP, fluorescence).
Typically, they are generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., affinity chromatography, fragmentation, label conjugation).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.