Thermo Fisher Scientific Rabbit anti-Donkey IgG (H+L) Secondary Antibody, DyLight 550
Rabbit에서 유래한 anti-Donkey IgG (H+L) DyLight 550 결합 2차 항체로, WB, IHC, ICC, Flow 등 다양한 응용에 사용 가능. 높은 특이성과 감도를 제공하며, 556/570 nm 파장에서 형광 검출. 항원 친화 크로마토그래피로 정제됨.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:2,000 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Donkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Donkey IgG-heavy and light chain |
| Conjugate | DyLight™ 550 |
| Excitation / Emission Max | 556 / 570 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556643 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Available Conjugate Formats
- AP
- Biotin
- DyLight 488
- DyLight 594
- DyLight 650
- DyLight 680
- DyLight 755
- DyLight 800
- FITC
- HRP
- Texas Red
- TRITC
- Custom conjugation available upon request
Target Information
Anti-Donkey secondary antibodies are affinity-purified antibodies with well-characterized specificity for equine (Donkey) immunoglobulins. They are useful for detection, sorting, or purification of their specified targets. Secondary antibodies provide versatility for various detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and may undergo further purification or modification (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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