Thermo Fisher Scientific Rabbit anti-Donkey IgG (H+L) Secondary Antibody, DyLight 550

Applications

Tested Dilution

Western Blot (WB)

1:5,000-1:20,000

Immunohistochemistry (IHC)

1:50-1:2,000

Immunocytochemistry (ICC/IF)

1:50-1:2,000

Flow Cytometry (Flow)

1:50-1:200

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Donkey

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Donkey IgG-heavy and light chain

Conjugate

DyLight™ 550 DyLight™ 550 DyLight™ 550

View additional formats

• [AP](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/AP/host/Rabbit/)
• [Biotin ](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/Biotin/host/Rabbit/)
• [DyLight 488](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/DyLight 488/host/Rabbit/)
• [DyLight 594](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/DyLight 594/host/Rabbit/)
• [DyLight 650](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/DyLight 650/host/Rabbit/)
• [DyLight 680](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/DyLight 680/host/Rabbit/)
• [DyLight 755](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/DyLight 755/host/Rabbit/)
• [DyLight 800](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/DyLight 800/host/Rabbit/)
• [FITC](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/FITC/host/Rabbit/)
• [HRP](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/HRP/host/Rabbit/)
• [Texas Red](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/Texas Red/host/Rabbit/)
• [TRITC](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/TRITC/host/Rabbit/)
• Request custom conjugation

Excitation/Emission Max

556/570 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 6.8 to 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2556643

Target

IgG

Antibody Form

Whole Antibody

Target Information

Anti-Donkey secondary antibodies are affinity-purified antibodies with well-characterized specificity for equine (Donkey) immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.