Thermo Fisher Scientific Rabbit anti-Donkey IgG (H+L) Secondary Antibody, DyLight 350

Applications

Tested Dilution

Western Blot (WB)

1:5,000-1:20,000

Immunohistochemistry (IHC)

1:50-1:2,000

Immunocytochemistry (ICC/IF)

1:50-1:2,000

Flow Cytometry (Flow)

1:25 - 1:100

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Donkey IgG-heavy and light chain

Conjugate

DyLight™ 350 DyLight™ 350 DyLight™ 350

View additional formats

• [AP](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/AP/host/Rabbit/)
• [Biotin ](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/Biotin/host/Rabbit/)
• [DyLight 488](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/DyLight 488/host/Rabbit/)
• [DyLight 550](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/DyLight 550/host/Rabbit/)
• [DyLight 594](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/DyLight 594/host/Rabbit/)
• [DyLight 650](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/DyLight 650/host/Rabbit/)
• [DyLight 680](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/DyLight 680/host/Rabbit/)
• [DyLight 755](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/DyLight 755/host/Rabbit/)
• [DyLight 800](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/DyLight 800/host/Rabbit/)
• [FITC](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/FITC/host/Rabbit/)
• [HRP](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/HRP/host/Rabbit/)
• [Texas Red](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/Texas Red/host/Rabbit/)
• [TRITC](/antibody/secondary/product-name/Donkey IgG (H--L)/conjugates/TRITC/host/Rabbit/)
• Request custom conjugation

Excitation/Emission Max

353/432 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2556641

Target

IgG

Antibody Form

Whole Antibody

Target Information

Anti-Donkey secondary antibodies are affinity-purified antibodies with well-characterized specificity for equine (Donkey) immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.