Thermo Fisher Scientific RAP1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

-

Immunocytochemistry (ICC/IF)

2-10 µg/mL

-

Immunoprecipitation (IP)

Assay-Dependent

-

Product Specifications

Species Reactivity

Gerbil, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues 212-235 of human Rap1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RAP1,uniProtId:Q9NYB0-1,ncbiNodeId:9606,antigenRange:212-235,antigenLength:399,antigenImageFileName:PA1-41267_RAP1_Q9NYB0-1_Rabbit.svg,antigenImageFileNamePDP:PA1-41267_RAP1_Q9NYB0-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1087032

Product Specific Information

Suggested positive control: 293 whole cell lysate, antigen standard for TERF2IP (transient overexpression lysate).

Target Information

Human Rap1 was the first identified ortholog of the yeast telomeric protein scRap1, which shares 3 conserved sequence motifs in common. HRap1 is located at telomeres and affects telomere length by being recruited to telomeres by TRF2 instead of directly binding to the telomere like scRap1. The hRap1 cDNA codes for a protein of 399 amino acids with approximate molecular weight of 47 kDa. The hRap1 protein does not interact with TRF1. TRF2 is a ubiquitously expressed protein that is implicated in the control of telomere length(1). TRF2, like TRF1 contains a Myb-related DNA binding motif. It binds to duplex TTAGGG repeats and is localized to all human telomeres in metaphase chromosomes. TRF2 is thought to protect chromosome ends by maintaining the correct structure at telomere termini. The use of mutant forms of TRF2 has implicated a role for TRF2 in the prevention of senescence in primary human cells. Recently, it has been shown that inhibition of TRF2 resulted in apoptosis in a subset of mammalian cell types.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.