Thermo Fisher Scientific E2F1 Polyclonal Antibody
E2F1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody입니다. 인간, 마우스, 랫트에 반응하며 WB, IHC, ICC, Flow, ELISA 등 다양한 응용에 적합합니다. 고순도 Protein A 정제, 1 mg/mL 농도, 연구용으로 사용됩니다.
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Thermo Fisher Scientific E2F1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human E2F1, amino acids 101–180 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of the cell cycle and the action of tumor suppressor proteins and is also a target of transforming proteins of small DNA tumor viruses.
E2F proteins contain several conserved domains:
- DNA binding domain
- Dimerization domain (interacts with DP proteins)
- Transactivation domain enriched in acidic amino acids
- Tumor suppressor protein association domain within the transactivation region
E2F1, along with E2F2 and E2F3, has an additional cyclin binding domain and binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: BS-0599R_E2F1_Q01094-1_Rabbit.svg, BS-0599R_E2F1_Q01094-1_Rabbit_PDP.jpeg)
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