Thermo Fisher Scientific Phospho-APC1 (Ser377) Polyclonal Antibody
Phospho-APC1 (Ser377) 단백질 인산화 부위를 인식하는 Rabbit Polyclonal 항체로, IHC, ICC/IF, ELISA 등에 사용 가능. Human, Mouse, Rat 반응성. Protein A 정제, -20°C 보관, 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human ANAPC1 around the phosphorylation site of Ser377 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Cell cycle regulated protein ubiquitination and degradation within subcellular domains is essential for normal mitosis progression.
APC1 (also known as mitotic checkpoint regulator, MCPR) is a conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase controlling progression through mitosis and the G1 phase.
APC/C mediates degradation of anaphase inhibitors, mitotic cyclins, and spindle-associated proteins, ensuring orderly mitotic events.
APC/C component mRNA and protein levels are expressed similarly across tissues and cell lines, indicating their function as part of a complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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