Thermo Fisher Scientific NWSHPQFEK Tag Monoclonal Antibody (5A9F9), FITC, THE

Applications

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1–3 µg/mL

Flow Cytometry (Flow)

• Tested Dilution: 1–3 µg per 1 × 10⁶ cells

Miscellaneous PubMed (Misc)

• Publications: View 1 publication

Product Specifications

Product Specific Information

Reconstitute the lyophilized product with deionized water (or equivalent) to make an antibody concentration of 0.5 mg/mL.

Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It is widely used in molecular biology protocols due to its strong affinity for biotin.
Strep II tag (NWSHPQFEK) is a nine-amino-acid peptide with high specificity and affinity toward streptavidin.
GenScript THE™ NWSHPQFEK Tag Antibody [FITC], mAb, Mouse is THE™ NWSHPQFEK Tag Antibody conjugated with FITC, suitable for detecting expression levels of NWSHPQFEK (Strep II tag) tagged fusion proteins.

Target Information

The Strep-tag II is an eight-residue minimal peptide sequence (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys) exhibiting intrinsic affinity toward streptavidin.
It can be fused to recombinant proteins for purification or detection purposes.
The original Strep-tag was a nine-amino-acid peptide allowing affinity purification via streptavidin columns but restricted to C-terminal fusion.
Strep-tag II was re-engineered to enable placement at both N- and C-termini of recombinant proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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