Thermo Fisher Scientific F(ab`)2-Rabbit anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, FITC

Applications

Tested Dilution

Immunocytochemistry (ICC/IF)

1:2000

Flow Cytometry (Flow)

1:25-1:100

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Purified murine IgG.

Conjugate

FITC FITC FITC

View additional formats

• [Alexa Fluor 350 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 350/host/Rabbit/)
• [Alexa Fluor 488 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 488/host/Rabbit/)
• [Alexa Fluor 555 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 555/host/Rabbit/)
• [Alexa Fluor 568 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 568/host/Rabbit/)
• [Alexa Fluor 594 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 594/host/Rabbit/)
• [Alexa Fluor 633 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 633/host/Rabbit/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 647/host/Rabbit/)
• [Alexa Fluor 680 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 680/host/Rabbit/)
• [AP](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/AP/host/Rabbit/)
• [Biotin ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Biotin/host/Rabbit/)
• [DyLight 488](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 488/host/Rabbit/)
• [DyLight 550](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 550/host/Rabbit/)
• [DyLight 650](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 650/host/Rabbit/)
• [DyLight 680](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 680/host/Rabbit/)
• [DyLight 755](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 755/host/Rabbit/)
• [DyLight 800](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 800/host/Rabbit/)
• [HRP](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/HRP/host/Rabbit/)
• [TRITC](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/TRITC/host/Rabbit/)
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_931521

Target

IgG

Cross Adsorption

Against Human serum proteins

Antibody Form

F(ab`)2 Fragment

Product Specific Information

Some cross reactivity with mouse IgM and IgA is expected, as is cross reactivity with rat IgG. Cross reactivity with human serum proteins has been minimized by solid phase adsorption. F(ab`)2 fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove the remaining intact IgG or Fc fragments.

For Flow Cytometry, use 50 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

FITC conjugated Rabbit F(ab`)2 anti Mouse IgG antibody recognizes all subclasses of mouse IgG.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.