Thermo Fisher Scientific TOP2A Monoclonal Antibody (OTI10G1), TrueMAB
Human TOP2A 단백질(aa 1100-1531)에 특이적인 Mouse monoclonal antibody. Western blot에 1:2,000 희석으로 검증됨. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도. 세포주기 후반기 단계 표지 및 종양세포 약물 내성 연구에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI10G1 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1100–1531 of human TOP2A produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Topo II alpha plays crucial roles in DNA synthesis, transcription, and chromosomal segregation during mitosis.
It serves as a sensitive and specific marker of late S-, G2-, and M-phases in transformed and developmentally regulated normal cells.
Additionally, Topo II alpha is implicated in tumor cell drug resistance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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