Thermo Fisher Scientific Calponin-1 Recombinant Mouse Monoclonal Antibody (rCNN1, 832)
Calponin-1 단백질을 표적하는 Thermo Fisher의 재조합 마우스 단일클론 항체. Western blot, IHC, Flow Cytometry 등 다양한 응용에 적합. 인간 및 랫트 반응성. Protein A/G 정제, 액상 형태로 4°C 보관.
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Thermo Fisher Scientific Calponin-1 Recombinant Mouse Monoclonal Antibody (rCNN1, 832)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rCNN1, 832 |
| Immunogen | Recombinant full-length human CNN1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Calponin is a thin filament-associated protein involved in regulating smooth muscle contraction. It binds to actin, calmodulin, troponin C, and tropomyosin. Calponin is expressed in smooth muscle and tissues with significant smooth muscle content.
Two isoforms exist (34 kDa and 29 kDa). The 29 kDa isoform is mainly found in urogenital muscle. Calponin expression is also observed in myoepithelial cells from benign and malignant breast lesions. It stains smooth muscle, myoepithelial cells, myofibroblasts, keratinocytes, and nerve fibers, aiding in differentiation of breast collagenous spherulosis (positive) from adenoid cystic carcinoma. Adenoid cystic carcinoma in salivary gland tumors is calponin positive.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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