Thermo Fisher Scientific Phospho-c-Myc (Thr358) Polyclonal Antibody

Thermo Fisher Scientific Phospho-c-Myc (Thr358) Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Suggested positive control for Western blot: HT29 cells
• Suggested positive control for IHC: Human breast carcinoma
• Suggested positive control for ICC/IF: HeLa cells

Target Information

The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is commonly activated in various tumor cells and plays a crucial role in cellular proliferation, differentiation, apoptosis, and cell cycle progression.

Phosphorylation of c-Myc at Thr58/Ser62 by kinases such as GSK3, CDK, ERK2, and JNK is functionally associated with cell proliferation and cycle regulation. c-Myc is essential for vasculogenesis and angiogenesis in tumor development.

The c-myc oncogene (p62 c-myc) regulates normal cellular proliferation and differentiation, while deregulated expression induces apoptosis. Antibodies against c-myc epitopes recognize Myc-tagged fusion proteins. Amplification of c-Myc is found in lung, breast, and colon carcinomas; N-Myc amplification occurs in neuroblastomas. Translocation of c-myc on chromosome 8 to immunoglobulin loci on chromosomes 14, 2, or 22 is seen in Burkitt’s lymphoma and other B-cell proliferative disorders. Aberrant c-myc expression is observed in various tumors including colorectal, gastric, hepatic, mammary, ovarian, pulmonary, and hematopoietic neoplasms.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.