Thermo Fisher Scientific BAF53A Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:300–1:10,000

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
This antibody has specificity for Human Actin-like protein 6A/ACTL6A/BAF53a.

Target Information

BAF53A is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature.
The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration, and chromatin remodeling.
This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to the SWI/SNF complex in S. cerevisiae and Drosophila. The latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression.
Together with beta-actin, it is required for maximal ATPase activity of BRG1 and for the association of the BAF complex with chromatin/matrix.
Three transcript variants that encode two different protein isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.