Thermo Fisher Scientific RIG-I Recombinant Rabbit Monoclonal Antibody (6K8P2)

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

ELISA

• Tested Dilution: 1 µg/mL

Product Specifications

Target Information

Retinoic acid-inducible gene I (RIG-I) is a pattern recognition receptor (PRR) involved in the recognition of viral double-stranded RNA (dsRNA). Together with MDA5, RIG-I detects viral dsRNA and activates the innate immune response. Both MDA5 and RIG-I are RNA helicases that perform overlapping yet distinct roles.

RIG-I is activated by dsRNAs lacking a 5'-triphosphate end and short dsRNAs, whereas MDA5 is activated by long dsRNAs. Once activated, both proteins signal through IPS-1, activating transcription factors NF-κB and IRF-3, which ultimately lead to apoptosis, cytokine signaling, and inflammation.

RIG-I is essential for signaling by influenza A and B viruses, human respiratory syncytial virus, paramyxoviruses, Japanese encephalitis virus, and West Nile virus. MicroRNA-146a has been implicated in feedback inhibition of RIG-I-dependent antiviral response by negatively regulating RIG-I targets TRAF6, IRAK1, and IRAK2. Recent evidence also suggests that RIG-I participates in the detection of cytosolic DNA through RNA polymerase III activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.