Thermo Fisher Scientific Human RC3H1 Synthetic Peptide

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

-

Blocking Assay (BLOCK)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Class

Synthetic

Type

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

purified

Storage buffer

PBS, pH 7.2, with 0.1% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

This peptide corresponds to 18 amino acid peptide near the carboxy terminus of human RC3H1.

PEP-1562 can be used as a blocking peptide with polyclonal antibody PA5-34519. For use as a blocking peptide in Western blot, incubate the peptide with equal volume of antibody for 30 min at 37ºC.

Target Information

The ring finger protein RC3H1, also known as Roquin, is a highly conserved member of the RING type ubiquitin ligase protein family whose M199R mutation leads to the excessive production of follicular helper T cells and germinal centers in the sanroque strain of mice, a strain with excessive IL-21 production and high titers of autoantibodies. The complete loss of RC3H1 induces early death and immune deregulation but not autoimmunity in RC3H1- mice, suggesting that the mutant RC3H1 is more disruptive to the immune system than its complete loss.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.