Thermo Fisher Scientific PD-L2 (PDCD1LG2) Monoclonal Antibody (OTI6C3), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended desalting product: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

Programmed death-ligand 2 (PD-L2, also known as B7-DC) belongs to the B7 ligand family within the immunoglobulin superfamily. Along with PD-L1, it acts as a ligand for programmed cell death protein 1 (PD-1).
PD-L2 is primarily expressed in dendritic cells but can be induced in various immune and non-immune cells depending on the microenvironment. Its expression is upregulated by cytokines such as IL-4, TNF-alpha, and IFN-gamma.

Although PD-L2 is generally expressed at lower levels than PD-L1, it has a 2–6 times higher affinity for PD-1. PD-1 and its ligands serve as inhibitory immune checkpoint molecules that regulate immune homeostasis by inhibiting T-cell activation, proliferation, and cytokine secretion (e.g., IFN-gamma, IL-10).

Interestingly, PD-L2 can also stimulate T-cell proliferation and cytokine production even in PD-1-deficient T cells, implying the existence of other receptors. Recent research identified repulsive guidance molecule b (RGMb) as a secondary receptor for PD-L2. RGMb, expressed in the central nervous system and macrophages, participates in regulating respiratory tolerance in the lung through BMP and/or neogenin signaling pathways.

The human PD-L2 monomer consists of:

• 201-amino-acid extracellular domain
• 21-amino-acid transmembrane domain
• 32-amino-acid cytoplasmic domain

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.