Thermo Fisher Scientific CD9 Monoclonal Antibody (MEM-61)
CD9 단백질의 EC2 도메인을 인식하는 Mouse IgG1 단클론 항체. Western blot, IHC, Flow cytometry 등 다양한 응용에 사용 가능. 인간 시료 반응성. 비결합형, PBS 용액 형태로 제공되며 4°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2–4 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 10 µg/mL | - |
| Flow Cytometry (Flow) | 1–4 µg/mL | View 1 publication |
| Functional Assay | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MEM-61 |
| Immunogen | Pre-B cell line NALM-6 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1073478 |
Available Conjugated Formats
Product Specific Information
This antibody recognizes an epitope on the second extracellular domain (EC2) of the CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes, and activated T lymphocytes.
It has also been successfully used in Mass Cytometry (CyTOF).
- Western Blot: non-reducing conditions
- Functional Application: Induction
Target Information
CD9 antigen is a glycoprotein expressed on developing B lymphocytes, platelets, monocytes, eosinophils, basophils, stimulated T lymphocytes, neurons, and glial cells in the peripheral nervous system.
It belongs to the tetraspanin family, characterized by four transmembrane domains.
CD9 regulates cell activation and aggregation, possibly through association with integrin CD41/CD61 (GPIIb/GPIIIa).
It is involved in various biological processes such as:
- Cell motility and adhesion
- Osteoclastogenesis and neurite outgrowth
- Myotube formation and sperm-egg fusion
- Regulation of cell proliferation and differentiation
CD9 also acts as a metastasis suppressor in solid tumors and plays a key role in immune cell interaction and signaling.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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