Thermo Fisher Scientific ICAM-1 (CD54) Polyclonal Antibody
인간 ICAM-1(CD54)에 특이적인 Goat Polyclonal 항체로 WB, ICC/IF, Flow Cytometry에 사용 가능. Extracellular domain 기반 면역원으로 높은 특이성과 감도를 제공. 액상 형태, TBS buffer 및 BSA 포함, -20°C 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.3 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide with sequence C-PNVILTKPEVSEGTE (aa 313–327) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2933603 |
Product Specific Information
- The immunizing peptide represents part of the extracellular domain and does not overlap any known glycosite.
- Tested in Peptide ELISA: antibody detection limit dilution 1:128,000.
Target Information
ICAM-1 (CD54) is an 85–110 kDa single-chain type I integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region, and a cytoplasmic domain.
ICAM-1 has seven potential N-linked glycosylation sites and shares sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102).
It binds to integrins CD11a/CD18 (LFA-1) and CD11b/CD18 (Mac-1), and serves as a receptor for Rhinovirus.
ICAM-1 is expressed on activated endothelial cells, epithelial cells, fibroblasts, chondrocytes, B and T lymphocytes, monocytes, macrophages, dendritic cells, and neutrophils. Expression increases upon inflammation and is also detected in some carcinoma and melanoma cells.
Soluble ICAM-1 is detectable in plasma and elevated in various inflammatory syndromes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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