Thermo Fisher Scientific p53 Monoclonal Antibody (OTI3A6), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nutrient deprivation.
p53 upregulates stress-response genes such as p21waf1, contributing to G1 or G2/M arrest. The structure includes an N-terminal transactivation domain, a central DNA-binding domain, an oligomerization domain, and a C-terminal regulatory domain.
Phosphorylation at Ser15 is critical for activation and stabilization, while phosphorylation at Ser392 is associated with tumor formation. p53 also plays roles in aging, oxidative stress, and neuronal functions such as long-term potentiation.
Mutations in TP53 are common in human cancers, leading to loss of tumor suppressor activity. Germline mutations are linked to cancer-prone syndromes such as Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.