Thermo Fisher Scientific EEF2 Recombinant Rabbit Monoclonal Antibody (4E2Z5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

4E2Z5

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 759-858 of human eEF2 (P13639). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EEF2,uniProtId:P13639-1,ncbiNodeId:9606,antigenRange:759-858,antigenLength:858,antigenImageFileName:MA5-42937_EEF2_P13639-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42937_EEF2_P13639-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2912078

Product Specific Information

Positive test controls include: HeLa, NIH/3T3, Jurkat, Mouse liver, Rat spleen. The target is usually found in the following locations: Cytoplasm, Nucleus.

Immunogen sequence: IYGVLNRKRG HVFEESQVAG TPMFVVKAYL PVNESFGFTA DLRSNTGGQA FPQCVFDHWQ ILPGDPFDNS SRPSQVVAET RKRKGLKEGI PALDNFLDKL

Target Information

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.