Thermo Fisher Scientific Nucleolin/NCL Polyclonal Antibody
Rabbit polyclonal antibody against human Nucleolin (NCL). Validated for IHC and IHC-P applications. Reacts with multiple species including human and mouse. Supplied as a liquid, unconjugated form with 50 µg/mL concentration. Recommended storage at 4°C.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:500 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 550 and the C-terminus (residue 710) of human Nucleolin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 50 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, predicted to react with Rat, Bovine, Dog, Horse, Rabbit, Guinea pig, Pig, Panda, Orangutan, Monkey, Gorilla, and Chimpanzee
Target Information
Nucleolin (NCL) is a eukaryotic nucleolar phosphoprotein involved in ribosome synthesis and maturation. It is mainly located in the dense fibrillar regions of the nucleolus. The human NCL gene contains 14 exons and 13 introns, spanning approximately 11 kb. Intron 11 encodes a small nucleolar RNA termed U20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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