Thermo Fisher Scientific Human ULK1 Synthetic Peptide

Thermo Fisher Scientific Human ULK1 Synthetic Peptide

Applications

• Control (Ctrl): Assay-dependent
• Blocking Assay (BLOCK): Assay-dependent

Product Specifications

Product Specific Information

This peptide corresponds to 16 amino acids near the center of human ULK1.
PEP-1585 can be used as a blocking peptide with polyclonal antibody PA5-34542.
For use as a blocking peptide in Western blot, incubate the peptide with equal volume of antibody for 30 min at 37ºC.

Target Information

ULK1 is a serine/threonine protein kinase that plays a critical role during the initial stages of autophagy, a vital cellular response to nutrient starvation.
The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein.
Knockdown of ULK1 inhibits the autophagic response and rapamycin-induced autophagy, consistent with its role downstream of mTOR.
ULK1 forms a complex with FIP200 and ATG13, essential for starvation-induced autophagy.
Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and for ULK1 protein stability.
ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.

Usage Note

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.