Thermo Fisher Scientific GAPDH Polyclonal Antibody
Thermo Fisher Scientific의 GAPDH Polyclonal Antibody는 Western blot, IHC, IP 등 다양한 응용에 적합한 토끼 유래 IgG 항체입니다. 고순도 액상 형태로 제공되며, 4°C에서 보관합니다. GAPDH 단백질 검출 및 로딩 컨트롤로 연구용에 최적화되어 있습니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 300 and the C-terminus (residue 333) of mouse Glyceraldehyde-3-Phosphate Dehydrogenase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Rat
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme involved in glycolysis. It exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphate in the presence of NADPH.
Beyond glycolysis, GAPDH participates in DNA replication, DNA repair, nuclear RNA export, membrane fusion, and microtubule bundling. It is implicated in apoptosis, neurodegenerative diseases, and cancer-related processes.
Due to its ubiquitous expression and high abundance, GAPDH is widely used as a protein loading control in Western blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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