Thermo Fisher Scientific GAPDH Polyclonal Antibody
Rabbit polyclonal antibody targeting human GAPDH, validated for Western blot. Recognizes residues 300–335 of GAPDH. Suitable as a protein loading control. Supplied as liquid, 0.2 mg/mL, stored at 4°C. For research use only.
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Applications
- Western Blot (WB): 1:2,000–1:10,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 300 and the C-terminus (residue 335) of human Glyceraldehyde-3-Phosphate Dehydrogenase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, predicted to react with Cat and Orangutan
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme involved in glycolysis. It exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphate in the presence of NADPH.
Beyond glycolysis, GAPDH participates in DNA replication, DNA repair, nuclear RNA export, membrane fusion, and microtubule bundling. It plays roles in apoptosis, neurodegenerative diseases, and cancer-related processes. GAPDH interacts with proteins such as amyloid precursor protein (linked to Alzheimer’s disease) and Huntingtin (linked to Huntington’s disease).
Due to its high expression in most tissues, GAPDH serves as a reliable protein loading control in Western blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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