Thermo Fisher Scientific Nestin Monoclonal Antibody (2Q178)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

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Immunohistochemistry (IHC)

-

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Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunohistochemistry (Frozen) (IHC (F))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:20-1:200

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Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2Q178

Immunogen

Full length native Nestin protein purified from embryonic rat spinal cord. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Nestin,uniProtId:P21263-1,ncbiNodeId:10114,antigenRange:1-1893,antigenLength:1893,antigenImageFileName:MA1-91657_Nestin_P21263-1_House_mouse.svg,antigenImageFileNamePDP:MA1-91657_Nestin_P21263-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

TBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2151007

Product Specific Information

For use in ICC applications, cells can be fixed with 4% paraformaldehyde buffered with 50mM sodium borate at p.H. 9.5. for IHC-P and IHC-Fr tissue can be fixed with 4% paraformaldehyde at pH 7.4 for light microscopy.

The predicted molecular weight of MA1-91657 is ~200kDa.

Target Information

Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.