Thermo Fisher Scientific Nestin Monoclonal Antibody (2Q178)
Nestin 단백질을 인식하는 Mouse monoclonal antibody (Clone 2Q178). 다양한 조직 염색 및 세포 면역염색에 사용 가능. 고순도 액상 형태로 제공되며, 연구용으로 신경 및 줄기세포 마커 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:20–1:200 | - |
| Immunohistochemistry (Frozen) (IHC-F) | 1:20–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:20–1:200 | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2Q178 |
| Immunogen | Full length native Nestin protein purified from embryonic rat spinal cord |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | TBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2151007 |
Product Specific Information
For ICC applications, cells can be fixed with 4% paraformaldehyde buffered with 50 mM sodium borate at pH 9.5.
For IHC-P and IHC-Fr, tissues can be fixed with 4% paraformaldehyde at pH 7.4 for light microscopy.
The predicted molecular weight of MA1-91657 is approximately 200 kDa.
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells.
It is widely used as a marker for stem/progenitor cells, glioma cells, and tumor endothelial cells.
Nestin serves as a superior angiogenic marker for evaluating neovascularity of endothelial cells in tumors.
Highly specific antibodies to human Nestin can aid in characterizing progenitor cell differentiation, enhancing therapeutic potential of neural stem/progenitor cells, and identifying neuroepithelial tumor cells.
Nestin expression has also been observed in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker in proliferating endothelial cells of colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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