Thermo Fisher Scientific TNF alpha Monoclonal Antibody (6E10)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.02 µg/mL

-

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

ELISA (ELISA)

3 µg/mL (coating)

View 1 publication 1 publication

Product Specifications

Published species

Pig

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

6E10

Immunogen

Recombinant porcine TNF alpha (Baculovirus-derived)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_223612

Product Specific Information

MP391 detects natural and recombinant TNF alpha, with a predicted molecular weight of approximately 25 kDa.

This is a carrier-free low endotoxin formulation, <1.0 EU/mg.

This product can be used as a coating antibody in a sandwich ELISA (100 µg/well) in combination with biotin-labeled TNFa monoclonal antibody (Product # M302B). For Western blot, this antibody has been successfully used in conjunction with 0.02 µg/mL Biotin-conjugated Goat-anti-Mouse IgG (Product # 31800), 0.05 µg/mL HRP-conjugated Streptavidin (Product # N100), and recombinant Porcine TNFa (Product # RPTNFAI), followed by visualization using SuperSignal™ West Pico Chemiluminescent Substrate (Product # 34080).

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.