Thermo Fisher Scientific WNT1 Polyclonal Antibody, Biotin, PeproTech
Biotin으로 결합된 Rabbit Polyclonal Anti-Human WNT1 항체로, Western blot 및 ELISA에 최적화됨. 고순도 재조합 Wnt-1 항원을 이용해 생산되었으며, 세포 신호전달 및 암 연구용으로 적합. -20°C에서 보관.
- 카탈로그번호
- 500-P250BT-500UG
- 판매단위
- pk
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Thermo Fisher Scientific WNT1 Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | Detection limit: 1.5–3.0 ng/lane (reducing/non-reducing) |
| ELISA | 0.25–1.0 µg/mL | Sandwich ELISA with capture antibody PeproTech 500-P250 |
| In vitro Assay (IV) | – |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human Wnt-1 (38.4 kDa) |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient |
Product Specific Information
AA Sequence of recombinant protein:
ANSSGRWWGI VNVASSTNLL TDSKSLQLVL EPSLQLLSRK QRRLIRQNPG ILHSVSGGLQ SAVRECKWQF RNRRWNCPTA PGPHLFGKIV NRGCRETAFI FAITSAGVTH SVARSCSEGS IESCTCDYRR RGPGGPDWHW GGCSDNIDFG RLFGREFVDS GEKGRDLRFL MNLHNNEAGR TTVFSEMRQE CKCHGMSGSC TVRTCWMRLP TLRAVGDVLR DRFDGASRVL YGNRGSNRAS RAELLRLEPE DPAHKPPSPH DLVYFEKSPN FCTYSGRLGT AGTAGRACNS SSPALDGCEL LCCGRGHRTR TQRVTERCNC TFHWCCHVSC RNCTHTRVLH ECLPreparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Wnt-1. Anti-Human Wnt-1-specific antibody was purified by affinity chromatography and then biotinylated.Sandwich ELISA:
To detect hWNT-1 by sandwich ELISA (100 µL/well), use 0.25–1.0 µg/mL of this antibody. In conjunction with PeproTech Polyclonal Anti-Human WNT-1 (500-P250) as a capture antibody, enables detection of 0.2–0.4 ng/well of Recombinant hWNT-1.Western Blot:
To detect hWNT-1 by Western blot, use 0.1–0.2 µg/mL. Detection limit for Recombinant hWNT-1 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.Package:
500-P250BT-1MG is provided as 2 × 500 µg.
Target Information
Wnt proteins are secreted glycoproteins that regulate tissue homeostasis, cell fate, proliferation, and self-renewal. Canonical Wnt signaling involves Frizzled and LRP5/LRP6 receptors, activating the β-catenin pathway, while non-canonical signaling involves Frizzled and ROR2 receptors. Dysregulation of these pathways can lead to cancer.
Human Wnt-1 is a 370 amino acid precursor cleaved to a 343 amino acid mature form (~42 kDa), showing ~99% identity to mouse Wnt-1. Wnt-1 signaling influences embryonic development and carcinogenesis and can induce phosphorylation and degradation of c-Myb.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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