Thermo Fisher Scientific WNT1 Polyclonal Antibody, Biotin, PeproTech
Rabbit 유래 Biotin 표지 WNT1 Polyclonal Antibody로 Western blot, ELISA, in vitro assay에 적합. 고순도 재조합 Human Wnt-1 항원으로 면역화되어 높은 특이성과 감도를 제공. 항원 친화 크로마토그래피로 정제되어 신뢰성 높은 결과 보장.
- 카탈로그번호
- 500-P250BT-xxxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific WNT1 Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.25–1.0 µg/mL | - |
| In vitro Assay (IV) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human Wnt-1 (38.4 kDa) |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930063 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
ANSSGRWWGI VNVASSTNLL TDSKSLQLVL EPSLQLLSRK QRRLIRQNPG ILHSVSGGLQ SAVRECKWQF RNRRWNCPTA PGPHLFGKIV NRGCRETAFI FAITSAGVTH SVARSCSEGS IESCTCDYRR RGPGGPDWHW GGCSDNIDFG RLFGREFVDS GEKGRDLRFL MNLHNNEAGR TTVFSEMRQE CKCHGMSGSC TVRTCWMRLP TLRAVGDVLR DRFDGASRVL YGNRGSNRAS RAELLRLEPE DPAHKPPSPH DLVYFEKSPN FCTYSGRLGT AGTAGRACNS SSPALDGCEL LCCGRGHRTR TQRVTERCNC TFHWCCHVSC RNCTHTRVLH ECL.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Wnt-1. The anti-Human Wnt-1-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect hWNT-1 by sandwich ELISA (100 µL/well antibody solution), use 0.25–1.0 µg/mL of this antibody.
When used with PeproTech Polyclonal Anti-Human WNT-1 (500-P250) as a capture antibody, it allows detection of at least 0.2–0.4 ng/well of Recombinant hWNT-1.
Western Blot:
For Western blot detection of hWNT-1, use 0.1–0.2 µg/mL of this antibody.
With compatible secondary reagents, the detection limit for Recombinant hWNT-1 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Packaging:
500-P250BT-1MG is provided as 2 × 500 µg.
Target Information
Wnt proteins are secreted glycoproteins that activate signaling pathways regulating tissue homeostasis, cell fate, proliferation, and self-renewal.
Canonical signaling begins when Wnt binds to a Frizzled receptor and LRP5/LRP6 coreceptor, activating the β-catenin pathway.
Non-canonical pathways involve Frizzled and ROR2 receptors.
Dysregulation of these pathways can lead to cancer.
Human Wnt-1 is a 370 amino acid precursor that is processed into a 343 amino acid (42 kDa) mature form.
Mature human Wnt-1 shares ~99% sequence identity with mouse Wnt-1.
Depending on tissue context, Wnt-1 can activate canonical and non-canonical pathways and is involved in embryonic development and carcinogenesis.
Wnt-1 signaling can also promote phosphorylation and degradation of c-Myb.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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