Thermo Fisher Scientific PIK3C2A Monoclonal Antibody (OTI4E6), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

The Class II PI3 kinases, including PIK3C2A, phosphorylate the 3 position of PI or PI4P. Although less studied than Class I PI3 kinases, Class II enzymes are involved in receptor-mediated cellular processes such as insulin signaling, neuronal survival, and growth factor signaling.
PI3-kinases contribute to pathways regulating cell proliferation, oncogenic transformation, cell survival, migration, and intracellular trafficking.
PIK3C2A contains a lipid kinase catalytic domain and a C-terminal C2 domain, typical of Class II PI3-kinases. C2 domains act as calcium-dependent phospholipid-binding motifs for membrane translocation and protein-protein interactions.
PI3-kinase activity of PIK3C2A is insensitive to nanomolar levels of wortmannin. It can be activated by insulin and may participate in integrin-dependent signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.