Thermo Fisher Scientific GARP Monoclonal Antibody (G14D9), Super Bright 702, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.5 µg)/Test
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Product Specifications

Additional Formats

• eFluor 660
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 600
• Request custom conjugation

Product Specific Information

Description:
The G14D9 monoclonal antibody reacts with human Glycoprotein A Repetitions Predominant (GARP, also known as LRRC32 or Garpin). GARP is an ~80 kDa glycoprotein expressed on the cell surface. Expression has been reported in placenta, lung, kidney, heart, ovary, liver, skeletal muscle, and pancreas. Protein expression is observed on megakaryocytes, platelets, and activated regulatory T (Treg) cells. GARP expression on activated Treg cells is necessary for their suppressive function, possibly related to its role as a receptor for LAP/TGF beta.

Applications Reported:
This G14D9 antibody is reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. Use at 5 µL (0.5 µg) per test (final volume 100 µL). Cell number may vary from 10⁵ to 10⁸ cells/test.

Fluorochrome Information:
Super Bright 702 is a tandem dye excited with the violet laser line (405 nm) and emits at 702 nm. Recommended detection with a 710/50 bandpass filter. Ensure instrument compatibility.

Staining Buffer Recommendation:
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Light Sensitivity:
Sensitive to photo-induced oxidation. Protect vial and stained samples from light.

Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency/compensation. Clone-specific performance should be verified empirically.

Excitation / Emission / Laser:
405 nm / 702 nm / Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

GARP (leucine rich repeat containing 32) is a type I membrane protein with twenty-two leucine-rich repeats and a short intracytoplasmic region. Identified via gene expression profiling of Treg and Th cells following TCR stimulation, GARP is expressed in CD4⁺, CD25⁺, FoxP3⁺ cells (Tregs) and may serve as a cell surface marker for Treg cells.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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