Thermo Scientific™

GeneBLAzer™ EDG3-Gα15 HEK 293T DA Assay Kit

This cell line is available as dividing cells by requesting a quote for K1234 from dividingcells@invitrogen.comGeneBLAzer™ EDG3-Gα15 HEK 293T DA자세히 알아보기

This cell line is available as dividing cells by requesting a quote for K1234 from dividingcells@invitrogen.com

GeneBLAzer™ EDG3-Gα15 HEK 293T DA (Division Arrested) cells and EDG3-Gα15-NFAT-bla HEK 293T cells contain the human EDG3 receptor stably integrated in the GeneBLAzer™ Gα15-NFAT-bla HEK 293T cell line. GeneBLAzer™ Gα15-NFAT-bla HEK 293T cells (Cat. No. K1212) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element, and stably express the promiscuous G-protein, Gα15. Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates.

DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both EDG3-Gα15 HEK 293T DA cells and EDG3-Gα15-NFAT-bla HEK 293T cells are functionally validated for Z`-factor and EC50 concentrations of Sphingosine-1-phosphate (S1P), (Figure 1). In addition, EDG3-Gα15-NFAT-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

EDG-3(Endothelial-differentiation-gene-3)/S1P-3(Sphingosine-1-Phosphate-3) is a Gq/Gi/Go/G13/G12 coupled GPCR. EDG-3 has been shown to be responsible for bradycardia that has been induced in clinical trials as a side effect of the immunosuppressive drug FTY720 that targets EDG-1. EDG-3 has also been shown to induce vasodilation in response to sphingosylphosphorylcholine (SPC), sphingosine-1-phosphate (S1P), and lysosulfatide (LSF). The EDG-3 Receptor has also been shown to play a cooperative or redundant role in angiogenesis with EDG-1

사양

등록 번호NM_005226.2

어세이 입력Cell-based beta lactamase reporter gene

셀 상태Division-Arrested Cells

검출 방법Fluorescence

유전자 ID(Entrez)1903

유전자 기호EDG3

개체군 상태Single Cell Clone Origin

제품라인GeneBLAzer™

수량1 plate

리포터 유전자BLA (Beta-Lactamase)

타겟 입력EDG3

세포주293T (HEK)

System TypeGeneBLAzer™

Druggable TargetGPCR

Unit SizeEach