Thermo Scientific™

GeneBLAzer™ EDNRA HEK 293T DA Assay Kit

This cell line is available as dividing cells by requesting a quote for Cat. No. K1223 from dividingcells@invitrogen.comGeneBLAzer™ EDNRA HEK자세히 알아보기

This cell line is available as dividing cells by requesting a quote for Cat. No. K1223 from dividingcells@invitrogen.com

GeneBLAzer™ EDNRA HEK 293T DA (Division Arrested) cells and EDNRA-NFAT-bla HEK 293T cells contain the human Endothelin type A (EDNRA) receptor stably integrated into the CellSensor™ NFAT-bla HEK 293T cell line. CellSensor™ NFAT-bla HEK 293T cells (Cat. No. K1129) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (NFAT). Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates.

DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both EDNRA HEK 293T DA cells and EDNRA-NFAT-bla HEK 293T cells are functionally validated for Z`-factor and EC50 concentrations of ET-1 (Figure 1). In addition, EDNRA-NFAT-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Endothelin receptor type A (ENDRA) mediates signaling of endothelin proteins that regulate several critical biological processes, including development and function of blood vessels, production of certain hormones, and stimulation of cell growth and division. Defects in EDNRA-mediated signaling have been implicated in a wide range of diseases, including cancer, hypertension, and cardiovascular disorders.

사양

등록 번호NM_001957

어세이 입력Cell-based beta lactamase reporter gene

셀 상태Division-Arrested Cells

검출 방법Fluorescence

유전자 ID(Entrez)1909

유전자 기호EDNRA

개체군 상태Single Cell Clone Origin

제품라인GeneBLAzer™

수량1 plate

리포터 유전자BLA (Beta-Lactamase)

타겟 입력EDNRA

세포주293T (HEK)

System TypeGeneBLAzer™

Druggable TargetGPCR

Unit SizeEach