Thermo Fisher Scientific RNF185 Polyclonal Antibody
Thermo Fisher Scientific의 RNF185 Polyclonal Antibody는 인간, 생쥐, 랫트 시료에 반응하며 Western blot과 ELISA에 적합합니다. 정제된 rabbit IgG 항체로, RNF185 단백질의 1-130 아미노산 서열을 면역원으로 사용하였습니다. 액상 형태이며 -20°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–130 of human RNF185 (NP_6894802) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.33 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.01% thimerosal |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2901293 |
Product Specific Information
- Positive Samples: Mouse testis
- Immunogen sequence:
MASKGPSASA SPENSSAGGP SGSSNGAGES GGQDSTFECN ICLDTAKDAV ISLCGHLFCW PCLHQWLETR PNRQVCPVCK AGISRDKVIP LYGRGSTGQQ DPREKTPPRP QGQRPEPENR GGFQGFGFGD
Target Information
RNF185 is an E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating Lys-63-linked polyubiquitination of BNIP1. It acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the ER for ubiquitination and subsequent proteasome-mediated degradation. RNF185 protects cells from ER stress-induced apoptosis and is responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway. It preferentially associates with the E2 enzymes UBE2J1 and UBE2J2.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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