Thermo Fisher Scientific Collagen Type IV Polyclonal Antibody, FITC
FITC로 표지된 Collagen Type IV 폴리클로날 항체로, WB, IHC, ELISA, IP 등 다양한 응용 가능. 인간 및 소 조직 반응성. 고순도 친화 크로마토그래피 정제. 형광 최대치 498/517 nm. 장기보관 시 -20°C에서 보관 권장.
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Thermo Fisher Scientific Collagen Type IV Polyclonal Antibody, FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:20,000–1:100,000 |
| Immunoprecipitation (IP) | 1:100 |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Bovine, Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-Collagen Type IV produced by repeated immunizations with Collagen Type IV from human and bovine placenta |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles; store in the dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- Restore with 0.05 mL of deionized water (or equivalent).
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only before immediate use.
- Expiration date: one year from restoration.
Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards.
This antibody reacts with most mammalian Type IV collagens and shows negligible cross-reactivity with Type I, II, III, V, and VI collagens.
Non-specific cross-reaction with other human serum or extracellular matrix proteins is negligible.
Target Information
Collagen IV is a major component of basement membranes in tissues such as kidneys, lungs, and skin.
It forms a network-like structure providing support and stability.
Composed of three alpha chains encoded by COL4A1, COL4A2, and COL4A3 genes.
Mutations in these genes can cause disorders like Alport syndrome and Goodpasture syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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