Thermo Fisher Scientific Na+/H+ Exchanger 1 (NHE-1) (extracellular) Polyclonal Antibody
Na+/H+ Exchanger 1(NHE-1) 단백질의 extracellular 부위를 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, Flow Cytometry에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS(pH 7.4) 1% BSA buffer에 보존. 연구용으로만 사용.
- 카탈로그번호
- ANX-010-xxxxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Na+/H+ Exchanger 1 (NHE-1) (extracellular) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:25 |
| Flow Cytometry (Flow) | 2.5 µg |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | (C)RERSIGDVTTAPSE, corresponding to amino acid residues 54-67 of rat NHE-1, 1st extracellular loop |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitution: 25 µL, 50 µL, or 0.2 mL double distilled water (DDW), depending on sample size.
- Ships as lyophilized powder at room temperature.
- Upon arrival, store at -20°C.
- Reconstituted solution can be stored at 4°C for up to 1 week.
- For longer storage, aliquot and store at -20°C.
- Avoid multiple freeze/thaw cycles.
- Centrifuge all antibody preparations before use (10,000 × g, 5 min).
Target Information
The Na⁺/H⁺ antiporter (NHE-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells. It is specifically inhibited by the diuretic drug amiloride and activated by various signals including growth factors, mitogens, neurotransmitters, and tumor promoters.
NHE-1 also acts as an anchor for actin filaments, maintaining cortical cytoskeleton integrity through interactions with ERM proteins (ezrin, radixin, moesin). It may play a structural role independent of ion exchange and has been implicated in hypertension.
At least two isoforms of NHE-1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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