Thermo Fisher Scientific Na+/H+ Exchanger 5 (NHE-5) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CAEKELPWKSGQGD, corresponding to amino acid residues 759-772 of rat NHE-5, Intracellular, C-terminus if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SLC9A5,uniProtId:Q9Z0X2-1,ncbiNodeId:10114,antigenRange:759-772,antigenLength:898,antigenImageFileName:ANX-005-200UL_SLC9A5_Q9Z0X2-1_Rabbit.svg,antigenImageFileNamePDP:ANX-005-200UL_SLC9A5_Q9Z0X2-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

Na+/H+ exchangers-1-6 (Na+/H+ antiporters, NHE-1-6) are integral membrane proteins that are expressed in most mammalian tissues, where they regulate intracellular pH and cell volume. NHEs mediate the secondary active extrusion of hydrogen (H+) ions out of cells in exchange for extracellular sodium (Na+). Excluding NHE-1, which is ubiquitously expressed, NHE isoforms 2-6 have distinct tissue- and cell type-dependent expression and inhibitiory characteristics by amiloride analogs. Human NHE-5 transcript (solute carrier family 9, isoform-5, SLC9A5) is an 896 amino acid protein that is expressed in brain, testis, spleen and skeletal muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.