Thermo Fisher Scientific TGF beta-1 Monoclonal Antibody (B11-4C3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

-

ELISA (ELISA)

1 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

B11-4C3

Immunogen

Full length human TGF beta-1 (Met1-Ser390) fused with c-terminal polyhistidine tag if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TGF beta-1,uniProtId:P01137-1,ncbiNodeId:9606,antigenRange:1-390,antigenLength:390,antigenImageFileName:MA1-169_TGF_beta-1_P01137-1_House_mouse.svg,antigenImageFileNamePDP:MA1-169_TGF_beta-1_P01137-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536871

Product Specific Information

MA1-169 has been successfully used as a coating antibody in a sandwich ELISA with Product # MA1-116 used as a detection antibody.

Target Information

TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP. TGF beta-1 helps regulates proliferation, differentiation, adhesion, migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer. The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.