Thermo Fisher Scientific Cdc34 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

ELISA

• Tested Dilution: 1:20,000

Product Specifications

Product Specific Information

CDC34 Polyclonal Antibody detects endogenous levels of CDC34 protein.

Target Information

CDC34 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.
In vitro, it catalyzes Lys-48-linked polyubiquitination and cooperates with the E2 UBCH5C and SCF (FBXW11) E3 ligase complex for polyubiquitination of NFKBIA, leading to its proteasomal degradation.
It performs ubiquitin chain elongation from UBE2D3-primed NFKBIA-linked ubiquitin.
CDC34 cooperates with the SCF (SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1.
It also contributes to degradation of CREM isoform ICERIIγ and ATF15, regulating cAMP-induced transcription during meiotic and mitotic cycles.
CDC34 is involved in G2/M phase regulation through ubiquitination and degradation of WEE1 kinase and β-catenin.
It is a target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.