Thermo Fisher Scientific TCR V alpha 3.2 Monoclonal Antibody (RR3-16), APC, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.25 µg/test
  View 2 publications

Product Specifications

Available Formats

• FITC
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This RR3-16 monoclonal antibody reacts with the mouse T cell receptor (TCR) V alpha 3.2 chain. The TCR is composed of alpha and beta chains, and specificity is determined by Va, Ja, Vb, Db, and Jb gene rearrangement.
The RR3-16 antibody recognizes the V alpha 3.2 chain on T cells from mouse strains bearing the b (e.g., C57BL/6) or c haplotype (e.g., SJL, SWR, NOD) in the Tcra gene complex.
The V alpha 3.2 chain is absent in mice with the a (e.g., Balb/c, AKR, C3H) and d (e.g., DBA/1, DBA/2) haplotypes.
It is more highly expressed on CD8+ T cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse lymph node cells. Use ≤ 0.25 µg/test (defined as staining a cell sample in 100 µL final volume).
  Cell number may range from 10⁵–10⁸ cells/test. Optimize titration for best performance.
• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

T cell receptors (TCRs) distinguish foreign from self-peptides presented by MHC class II molecules, essential for adaptive immune response.
Self-peptide recognition is linked to autoimmune disease, while mutant self-peptides can be tumor-associated.
Superantigen engagement of TCRs can cause toxic shock syndrome.
Autoantibodies to TCR V beta segments have been found in rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE), potentially modulating TH1-mediated inflammation.
Most human T cells express alpha-beta TCRs along with CD4 or CD8.
A small subset lacks both (double negative, DN), often elevated in autoimmune or immunodeficiency disorders.
Gamma-delta T cells, found mainly in epithelia, show less diversity and distinct antigen recognition, with some subsets exhibiting antitumor and immunoregulatory activity.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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