Thermo Fisher Scientific ERCC1 Monoclonal Antibody (1E5B3)
ERCC1 단백질을 인식하는 Mouse 모노클로날 항체로, Western blot, ICC/IF, Flow cytometry, ELISA 등에 사용 가능. Human ERCC1(aa 151–297) 항원을 기반으로 제작되었으며, Protein G로 정제된 액상 형태. 연구용으로 DNA 손상 복구 기전 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1E5B3 |
| Immunogen | Purified recombinant fragment of human ERCC1 (aa 151–297) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, keep at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898429 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
ERCC1 is a non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5′ incision during DNA repair. It acts with SLX4 in the repair of interstrand cross-links (ICL) and participates in processing anaphase bridge-generating DNA structures that can lead to cytokinesis failure. ERCC1 is also required for homology-directed repair (HDR) of DNA double-strand breaks in conjunction with SLX4. (Source: Uniprot)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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