Thermo Fisher Scientific HSP70 Monoclonal Antibody (7FB), APC
Drosophila HSP70 단백질을 인식하는 rat monoclonal antibody(7FB)로, APC 형광 표지. Western blot, ICC/IF, ELISA에 사용 가능하며 70kDa 단백질 검출에 적합. Protein G 정제, 1 mg/mL 농도, 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:2,000 | |
| Immunocytochemistry (ICC/IF) | Assay-dependent | |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Fruit fly |
| Host / Isotype | Rat / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7FB |
| Immunogen | Prepared from Drosophila tissue culture cells heat shocked at 36.5°C for 3 hours, and isolated using SDS-PAGE. |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931785 |
Available Formats
- Unconjugated (MA5-27627)
- FITC (MA5-45332)
- PE (MA5-45334)
- PerCP (MA5-45333)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45331 was sufficient for detection of Drosophila HSP70 using an indirect assay with rabbit anti-rat IgG and goat anti-rabbit IgG:HRP.
Detects approximately 70 kDa (heat-inducible form).
Target Information
HSP70 is a heat shock protein (HSP) expressed in response to various biological stresses, including high temperatures.
The HSP70 family includes several conserved proteins induced by stress in all examined organisms.
Human HSP70 family members include:
- HSP70: strongly inducible in all organisms, constitutively expressed in primate cells
- HSP72: induced exclusively under stress conditions
- HSC70: constitutively expressed, involved in uncoating of clathrin-coated vesicles
- GRP78 (BiP): glucose-regulated, localized in the endoplasmic reticulum
- p75 (HSP75): mitochondrial localization
HSP70 stabilizes existing proteins against aggregation and assists folding of newly translated proteins.
It also participates in the ubiquitin–proteasome pathway via interaction with AU-rich element RNA-binding protein 1.
The HSP70 gene is located in the MHC class III region, clustered with two related genes encoding similar proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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