Thermo Scientific™

Maxima H Minus Reverse Transcriptase (200 U/μL)

Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses자세히 알아보기

Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT. The engineered enzyme features dramatically improved thermostability, 50X higher processivity, robustness, and increased synthesis rate compared to wild type M-MuLV RT.

The eliminated RNase H activity enables the enzyme to produce very long RNA transcripts up to 20 kb. Due to its high thermostability, the enzyme maintains full activity during the entire reverse transcription reaction and generates high yields of cDNA. The reaction temperature can be increased to 65°C for efficient transcription of RNA regions with a high secondary structure or to improve specificity using gene specific primers. The extremely high processivity of Maxima H Minus enzyme results in increased resistance to common reaction inhibitors, such as guanidine, formamide, and ethanol.

Features of Maxima H Minus Reverse Transcriptase include:
• Thermostable—90% active after incubation at 50°C for 60 minutes in a reaction mixture
• Active up to 65°C
• High yields of full-length cDNA up to 20 kb
• High sensitivity—reproducible cDNA synthesis from a wide range of starting total RNA amounts (1 pg to 5 μg)
• Efficient—complete cDNA synthesis in 15 to 30 minutes
• Increased resistance to common reaction inhibitors
• Incorporates modified nucleotides

Applications
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Reverse transcription at elevated temperatures to reduce effects of secondary structure
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• Analysis of RNA by primer extension

Related products
• Maxima H Minus Reverse Transcriptase
• Maxima H Minus First Strand cDNA Synthesis Kit
• Maxima H Minus First Strand cDNA Synthesis Kit, with dsDNase
• Maxima H Minus cDNA Synthesis Master Mix
• Maxima H Minus cDNA Synthesis Master Mix, with dsDNase

사양

형식Stand-alone Enzyme

GC-Rich PCR PerformanceHigh

반응 속도15 to 30 min.

기술Reverse Transcription

최적 반응 온도50°C to 55°C

수량2,000 Units

역전사 효소Maxima H Minus

리보뉴클레아제 H 활성Reduced

배송 조건Dry Ice

농도200 U/μL

최종 제품 유형First-Strand cDNA

반응 형식Separate components

시약 유형Reverse Transcription

크기(최종 제품)Up to 20 kb

시작 물질RNA

Unit SizeEach